Optical Coherence Tomography

Optical coherence tomography (OCT) is a high-resolution optical imaging technique. By using this technique, we can capture micrometer-resolution images in 3D from within the tissue. In the ophthalmology OCT is been used for a while and we would like to introduce the benefits of this technique in our field of research as well.

Optical coherence tomography (OCT) is the optical analog of ultrasound. It provides cross-sectional images of tissue by measuring the strength of backscattered light as a function of depth in tissue with a resolution of approximately 5-30 µm and a penetration depth of about 2-3 mm in tissue. Allowing three-dimensional imaging at (sub)cellular level,  OCT might improve the detection rates of for example, esophageal adenocarcinoma. Given its limited penetration depth, endoscopic use of OCT is needed to access the esophagus for imaging purposes.
To overcome the lacks in molecular imaging contrast of OCT, we combined it with fluorescence-based molecular imaging together with the VU Amsterdam (prof. Dr. Johannes de Boer). In fact, the development of novel near infrared fluorophores has opened the door to the possibility of fluorescently labelling a variety of monoclonal antibodies for targeted imaging.
The goal is to achieve molecular contrast combined with the structural OCT information to provide a paradigm shift in the kind of information available from minimally invasive imaging, approaching immunohistochemistry in vivo.
OCT_Histology
A reconstructed histology image. The green pixels show a high fluorescence intensity.

One of the first studies done with OCT by Prof. Dr. Johannes de Boer is described in this article: High resolution combined molecular and structural optical imaging of colorectal cancer in a xenograft mouse model (2018)